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Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells
Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762678/ https://www.ncbi.nlm.nih.gov/pubmed/29321585 http://dx.doi.org/10.1038/s41598-017-18568-4 |