Integrated sequencing of exome and mRNA of large-sized single cells

Current approaches of single cell DNA-RNA integrated sequencing are difficult to call SNPs, because a large amount of DNA and RNA is lost during DNA-RNA separation. Here, we performed simultaneous single-cell exome and transcriptome sequencing on individual mouse oocytes. Using microinjection, we ke...

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Detalles Bibliográficos
Autores principales: Wang, Lily Yan, Guo, Jiajie, Cao, Wei, Zhang, Meng, He, Jiankui, Li, Zhoufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762704/
https://www.ncbi.nlm.nih.gov/pubmed/29321653
http://dx.doi.org/10.1038/s41598-017-18730-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762704/
https://www.ncbi.nlm.nih.gov/pubmed/29321653
http://dx.doi.org/10.1038/s41598-017-18730-y

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