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Subcellular western blotting of single cells

Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors. To enhance selectivity while providing single-cell resolution, we introduce a subcellular western b...

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Detalles Bibliográficos
Autores principales: Yamauchi, Kevin A., Herr, Amy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764185/
https://www.ncbi.nlm.nih.gov/pubmed/29333327
http://dx.doi.org/10.1038/micronano.2016.79