Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

In this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice. The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide RNA. This simple cloning-free metho...

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Detalles Bibliográficos
Autores principales: Teixeira, Marie, Py, Bénédicte F., Bosc, Christophe, Laubreton, Daphné, Moutin, Marie-Jo, Marvel, Jacqueline, Flamant, Frédéric, Markossian, Suzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764989/
https://www.ncbi.nlm.nih.gov/pubmed/29323173
http://dx.doi.org/10.1038/s41598-017-18826-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764989/
https://www.ncbi.nlm.nih.gov/pubmed/29323173
http://dx.doi.org/10.1038/s41598-017-18826-5

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