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A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila
Heterotrimeric G proteins play central roles in many signaling pathways, including the phototransduction cascade in animals. However, the degree of involvement of the G protein subunit Gα(q) is not clear since animals with previously reported strong loss-of-function mutations remain responsive to li...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765363/ https://www.ncbi.nlm.nih.gov/pubmed/29158337 http://dx.doi.org/10.1534/g3.117.300340 |
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author | Cao, Jinguo Bollepalli, Murali K. Hu, Yuhui Zhang, Jin Li, Qiang Li, Hongmei Chang, Hua Xiao, Feng Hardie, Roger C. Rong, Yikang S. Hu, Wen |
author_facet | Cao, Jinguo Bollepalli, Murali K. Hu, Yuhui Zhang, Jin Li, Qiang Li, Hongmei Chang, Hua Xiao, Feng Hardie, Roger C. Rong, Yikang S. Hu, Wen |
author_sort | Cao, Jinguo |
collection | PubMed |
description | Heterotrimeric G proteins play central roles in many signaling pathways, including the phototransduction cascade in animals. However, the degree of involvement of the G protein subunit Gα(q) is not clear since animals with previously reported strong loss-of-function mutations remain responsive to light stimuli. We recovered a new allele of Gα(q) in Drosophila that abolishes light response in a conventional electroretinogram assay, and reduces sensitivity in whole-cell recordings of dissociated cells by at least five orders of magnitude. In addition, mutant eyes demonstrate a rapid rate of degeneration in the presence of light. Our new allele is likely the strongest hypomorph described to date. Interestingly, the mutant protein is produced in the eyes but carries a single amino acid change of a conserved hydrophobic residue that has been assigned to the interface of interaction between Gα(q) and its downstream effector, PLC. Our study has thus uncovered possibly the first point mutation that specifically affects this interaction in vivo. |
format | Online Article Text |
id | pubmed-5765363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-57653632018-01-12 A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila Cao, Jinguo Bollepalli, Murali K. Hu, Yuhui Zhang, Jin Li, Qiang Li, Hongmei Chang, Hua Xiao, Feng Hardie, Roger C. Rong, Yikang S. Hu, Wen G3 (Bethesda) Investigations Heterotrimeric G proteins play central roles in many signaling pathways, including the phototransduction cascade in animals. However, the degree of involvement of the G protein subunit Gα(q) is not clear since animals with previously reported strong loss-of-function mutations remain responsive to light stimuli. We recovered a new allele of Gα(q) in Drosophila that abolishes light response in a conventional electroretinogram assay, and reduces sensitivity in whole-cell recordings of dissociated cells by at least five orders of magnitude. In addition, mutant eyes demonstrate a rapid rate of degeneration in the presence of light. Our new allele is likely the strongest hypomorph described to date. Interestingly, the mutant protein is produced in the eyes but carries a single amino acid change of a conserved hydrophobic residue that has been assigned to the interface of interaction between Gα(q) and its downstream effector, PLC. Our study has thus uncovered possibly the first point mutation that specifically affects this interaction in vivo. Genetics Society of America 2017-11-20 /pmc/articles/PMC5765363/ /pubmed/29158337 http://dx.doi.org/10.1534/g3.117.300340 Text en Copyright © 2018 Cao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Cao, Jinguo Bollepalli, Murali K. Hu, Yuhui Zhang, Jin Li, Qiang Li, Hongmei Chang, Hua Xiao, Feng Hardie, Roger C. Rong, Yikang S. Hu, Wen A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title | A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title_full | A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title_fullStr | A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title_full_unstemmed | A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title_short | A Single Residue Mutation in the Gα(q) Subunit of the G Protein Complex Causes Blindness in Drosophila |
title_sort | single residue mutation in the gα(q) subunit of the g protein complex causes blindness in drosophila |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765363/ https://www.ncbi.nlm.nih.gov/pubmed/29158337 http://dx.doi.org/10.1534/g3.117.300340 |
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