Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation

Faithful expression of transgenes in cell cultures and mice is often challenged by locus dependent epigenetic silencing. We investigated silencing of Tet-controlled expression cassettes within the mouse ROSA26 locus. We observed pronounced DNA methylation of the Tet promoter concomitant with loss of...

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Detalles Bibliográficos
Autores principales: Gödecke, Natascha, Zha, Lisha, Spencer, Shawal, Behme, Sara, Riemer, Pamela, Rehli, Michael, Hauser, Hansjörg, Wirth, Dagmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766184/
https://www.ncbi.nlm.nih.gov/pubmed/28934472
http://dx.doi.org/10.1093/nar/gkx601

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766184/
https://www.ncbi.nlm.nih.gov/pubmed/28934472
http://dx.doi.org/10.1093/nar/gkx601

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