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Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation
Faithful expression of transgenes in cell cultures and mice is often challenged by locus dependent epigenetic silencing. We investigated silencing of Tet-controlled expression cassettes within the mouse ROSA26 locus. We observed pronounced DNA methylation of the Tet promoter concomitant with loss of...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766184/ https://www.ncbi.nlm.nih.gov/pubmed/28934472 http://dx.doi.org/10.1093/nar/gkx601 |