The production of UL16-binding protein 1 targeted pigs using CRISPR technology

Two sgRNAs were designed to target the region of exon 2 of the pULBP1 gene by microinjection. The co-injection of modified Cas9-D10A nickase with a pair of sgRNAs into the zygote’s cytoplasm easily and efficiently generated biallelic modification of the pULBP1 gene in one step. Five out of nine F0 g...

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Detalles Bibliográficos
Autores principales: Joanna, Zeyland, Magdalena, Hryhorowicz, Agnieszka, Nowak-Terpiłowska, Jacek, Jura, Ryszard, Słomski, Zdzisław, Smorąg, Barbara, Gajda, Daniel, Lipiński
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766454/
https://www.ncbi.nlm.nih.gov/pubmed/29354381
http://dx.doi.org/10.1007/s13205-018-1107-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766454/
https://www.ncbi.nlm.nih.gov/pubmed/29354381
http://dx.doi.org/10.1007/s13205-018-1107-4

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