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Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions

BACKGROUND: The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and i...

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Detalles Bibliográficos
Autores principales: Popovic, Milica, Mazzega, Elisa, Toffoletto, Barbara, de Marco, Ario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766977/
https://www.ncbi.nlm.nih.gov/pubmed/29331148
http://dx.doi.org/10.1186/s12934-017-0856-9