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A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids

Gut epithelial organoids are routinely used to investigate intestinal biology; however, current culture methods are not amenable to genetic manipulation, and it is difficult to generate sufficient numbers for high-throughput studies. Here, we present an improved culture system of human induced pluri...

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Detalles Bibliográficos
Autores principales: Takahashi, Yu, Sato, Shintaro, Kurashima, Yosuke, Yamamoto, Tomohisa, Kurokawa, Shiho, Yuki, Yoshikazu, Takemura, Naoki, Uematsu, Satoshi, Lai, Chen-Yi, Otsu, Makoto, Matsuno, Hiroshi, Osawa, Hideki, Mizushima, Tsunekazu, Nishimura, Junichi, Hayashi, Mikio, Yamaguchi, Takayuki, Kiyono, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768885/
https://www.ncbi.nlm.nih.gov/pubmed/29233552
http://dx.doi.org/10.1016/j.stemcr.2017.11.004