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A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids
Gut epithelial organoids are routinely used to investigate intestinal biology; however, current culture methods are not amenable to genetic manipulation, and it is difficult to generate sufficient numbers for high-throughput studies. Here, we present an improved culture system of human induced pluri...
Autores principales: | Takahashi, Yu, Sato, Shintaro, Kurashima, Yosuke, Yamamoto, Tomohisa, Kurokawa, Shiho, Yuki, Yoshikazu, Takemura, Naoki, Uematsu, Satoshi, Lai, Chen-Yi, Otsu, Makoto, Matsuno, Hiroshi, Osawa, Hideki, Mizushima, Tsunekazu, Nishimura, Junichi, Hayashi, Mikio, Yamaguchi, Takayuki, Kiyono, Hiroshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768885/ https://www.ncbi.nlm.nih.gov/pubmed/29233552 http://dx.doi.org/10.1016/j.stemcr.2017.11.004 |
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