A lentiviral system for efficient knockdown of proteins in neuronal cultures [version 1; referees: 2 approved]

We have devised a protocol for highly efficient and specific knockdown of proteins in neuronal cultures. Small hairpin RNAs (shRNAs) are embedded into a microRNA (miRNA) context by oligo annealing to create shRNAmiRs, which are expressed from within the 3′-UTR of a reporter protein. This reporter pr...

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Detalles Bibliográficos
Autores principales: Ritter, Brigitte, Ferguson, Shawn M., De Camilli, Pietro, McPherson, Peter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771425/
https://www.ncbi.nlm.nih.gov/pubmed/29355247

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771425/
https://www.ncbi.nlm.nih.gov/pubmed/29355247

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