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What can be observed in real time PCR and when does it show?

Real time, or quantitative, PCR typically starts from a very low concentration of initial DNA strands. During iterations the numbers increase, first essentially by doubling, later predominantly in a linear way. Observation of the number of DNA molecules in the experiment becomes possible only when i...

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Detalles Bibliográficos
Autores principales: Chigansky, Pavel, Jagers, Peter, Klebaner, Fima C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772144/
https://www.ncbi.nlm.nih.gov/pubmed/28667372
http://dx.doi.org/10.1007/s00285-017-1154-1