Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis

Lactococcus lactis is a food-grade lactic acid bacterium that is used in the dairy industry as a cell factory and as a host for recombinant protein expression. The nisin-controlled inducible expression (NICE) system is frequently applied in L. lactis; however new tools for its genetic modification a...

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Detalles Bibliográficos
Autores principales: Berlec, Aleš, Škrlec, Katja, Kocjan, Janja, Olenic, Maria, Štrukelj, Borut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772564/
https://www.ncbi.nlm.nih.gov/pubmed/29343791
http://dx.doi.org/10.1038/s41598-018-19402-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772564/
https://www.ncbi.nlm.nih.gov/pubmed/29343791
http://dx.doi.org/10.1038/s41598-018-19402-1

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