A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis

Engineered nucleases in genome editing manifest diverse efficiencies at different targeted loci. There is therefore a constant need to evaluate the mutation rates at given loci. T7 endonuclease 1 (T7E1) and Surveyor mismatch cleavage assays are the most widely used methods, but they are labour and t...

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Detalles Bibliográficos
Autores principales: Zhang, Hongmin, Zhou, Yuexin, Wang, Yinan, Zhao, Yige, Qiu, Yeting, Zhang, Xinyi, Yue, Di, Zhou, Zhuo, Wei, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773543/
https://www.ncbi.nlm.nih.gov/pubmed/29348585
http://dx.doi.org/10.1038/s41598-018-19317-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773543/
https://www.ncbi.nlm.nih.gov/pubmed/29348585
http://dx.doi.org/10.1038/s41598-018-19317-x

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