Improved in Vitro Folding of the Y(2) G Protein-Coupled Receptor into Bicelles

Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts of protein. Here, we present an improved protocol for Escherichia coli expression, functional refolding, and reconstitut...

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Detalles Bibliográficos
Autores principales: Schmidt, Peter, Bender, Brian J., Kaiser, Anette, Gulati, Khushboo, Scheidt, Holger A., Hamm, Heidi E., Meiler, Jens, Beck-Sickinger, Annette G., Huster, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776092/
https://www.ncbi.nlm.nih.gov/pubmed/29387686
http://dx.doi.org/10.3389/fmolb.2017.00100

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776092/
https://www.ncbi.nlm.nih.gov/pubmed/29387686
http://dx.doi.org/10.3389/fmolb.2017.00100

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