Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving specificity by incorporating chemical modification...

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Detalles Bibliográficos
Autores principales: Ryan, Daniel E, Taussig, David, Steinfeld, Israel, Phadnis, Smruti M, Lunstad, Benjamin D, Singh, Madhurima, Vuong, Xuan, Okochi, Kenji D, McCaffrey, Ryan, Olesiak, Magdalena, Roy, Subhadeep, Yung, Chong Wing, Curry, Bo, Sampson, Jeffrey R, Bruhn, Laurakay, Dellinger, Douglas J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778453/
https://www.ncbi.nlm.nih.gov/pubmed/29216382
http://dx.doi.org/10.1093/nar/gkx1199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778453/
https://www.ncbi.nlm.nih.gov/pubmed/29216382
http://dx.doi.org/10.1093/nar/gkx1199

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