Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Yi, Hu, Dehong, Markillie, Lye Meng, Chrisler, William B, Gaffrey, Matthew J, Ansong, Charles, Sussel, Lori, Orr, Galya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778465/
https://www.ncbi.nlm.nih.gov/pubmed/29040675
http://dx.doi.org/10.1093/nar/gkx874

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778465/
https://www.ncbi.nlm.nih.gov/pubmed/29040675
http://dx.doi.org/10.1093/nar/gkx874

Ejemplares similares