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Fluorescently-tagged human eIF3 for single-molecule spectroscopy

Human translation initiation relies on the combined activities of numerous ribosome-associated eukaryotic initiation factors (eIFs). The largest factor, eIF3, is an ∼800 kDa multiprotein complex that orchestrates a network of interactions with the small 40S ribosomal subunit, other eIFs, and mRNA, w...

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Detalles Bibliográficos
Autores principales: Johnson, Alex G, Petrov, Alexey N, Fuchs, Gabriele, Majzoub, Karim, Grosely, Rosslyn, Choi, Junhong, Puglisi, Joseph D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778468/
https://www.ncbi.nlm.nih.gov/pubmed/29136179
http://dx.doi.org/10.1093/nar/gkx1050