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Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry

Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its ‘melting point’. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a micr...

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Detalles Bibliográficos
Autores principales: Hoeser, Jo, Gnandt, Emmanuel, Friedrich, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780519/
https://www.ncbi.nlm.nih.gov/pubmed/29362420
http://dx.doi.org/10.1038/s41598-018-19702-6