Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry

Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its ‘melting point’. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a micr...

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Detalles Bibliográficos
Autores principales: Hoeser, Jo, Gnandt, Emmanuel, Friedrich, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780519/
https://www.ncbi.nlm.nih.gov/pubmed/29362420
http://dx.doi.org/10.1038/s41598-018-19702-6
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author Hoeser, Jo
Gnandt, Emmanuel
Friedrich, Thorsten
author_facet Hoeser, Jo
Gnandt, Emmanuel
Friedrich, Thorsten
author_sort Hoeser, Jo
collection PubMed
description Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its ‘melting point’. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the ‘melting point’ of soluble and membranous proteins at various buffer conditions.
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spelling pubmed-57805192018-02-06 Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry Hoeser, Jo Gnandt, Emmanuel Friedrich, Thorsten Sci Rep Article Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its ‘melting point’. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the ‘melting point’ of soluble and membranous proteins at various buffer conditions. Nature Publishing Group UK 2018-01-23 /pmc/articles/PMC5780519/ /pubmed/29362420 http://dx.doi.org/10.1038/s41598-018-19702-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hoeser, Jo
Gnandt, Emmanuel
Friedrich, Thorsten
Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title_full Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title_fullStr Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title_full_unstemmed Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title_short Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
title_sort low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780519/
https://www.ncbi.nlm.nih.gov/pubmed/29362420
http://dx.doi.org/10.1038/s41598-018-19702-6
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AT friedrichthorsten lowcostmicrocontrollerbasedheatingdeviceformultiwavelengthdifferentialscanningfluorimetry

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