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Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry
Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its ‘melting point’. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a micr...
Autores principales: | Hoeser, Jo, Gnandt, Emmanuel, Friedrich, Thorsten |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780519/ https://www.ncbi.nlm.nih.gov/pubmed/29362420 http://dx.doi.org/10.1038/s41598-018-19702-6 |
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