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MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782377/ https://www.ncbi.nlm.nih.gov/pubmed/29368647 http://dx.doi.org/10.1186/s12943-018-0768-2 |
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author | Huang, Wei Wang, Wen-Tao Fang, Ke Chen, Zhen-Hua Sun, Yu-Meng Han, Cai Sun, Lin-Yu Luo, Xue-Qun Chen, Yue-Qin |
author_facet | Huang, Wei Wang, Wen-Tao Fang, Ke Chen, Zhen-Hua Sun, Yu-Meng Han, Cai Sun, Lin-Yu Luo, Xue-Qun Chen, Yue-Qin |
author_sort | Huang, Wei |
collection | PubMed |
description | Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane protein that inhibits phagocytosis in T cell acute lymphoblastic leukemia. miR-708 directly targeted CD47 through binding to 3’UTR and is inversely correlated with CD47 expression. Functional studies showed that restoration of miR-708 expression in the T-ALL cell line is sufficient to promote phagocytosis by macrophages in the absence or presence of the anti-CD47 antibody to eradicate T-ALL cells, and inhibited tumor engraftment in vivo. Together, our findings suggest that miR-708 is a key negative regulator of CD47 and may serve as an attractive candidate for immunotherapy of T-ALL. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-018-0768-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5782377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57823772018-02-06 MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 Huang, Wei Wang, Wen-Tao Fang, Ke Chen, Zhen-Hua Sun, Yu-Meng Han, Cai Sun, Lin-Yu Luo, Xue-Qun Chen, Yue-Qin Mol Cancer Letter to the Editor Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane protein that inhibits phagocytosis in T cell acute lymphoblastic leukemia. miR-708 directly targeted CD47 through binding to 3’UTR and is inversely correlated with CD47 expression. Functional studies showed that restoration of miR-708 expression in the T-ALL cell line is sufficient to promote phagocytosis by macrophages in the absence or presence of the anti-CD47 antibody to eradicate T-ALL cells, and inhibited tumor engraftment in vivo. Together, our findings suggest that miR-708 is a key negative regulator of CD47 and may serve as an attractive candidate for immunotherapy of T-ALL. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-018-0768-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-24 /pmc/articles/PMC5782377/ /pubmed/29368647 http://dx.doi.org/10.1186/s12943-018-0768-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Letter to the Editor Huang, Wei Wang, Wen-Tao Fang, Ke Chen, Zhen-Hua Sun, Yu-Meng Han, Cai Sun, Lin-Yu Luo, Xue-Qun Chen, Yue-Qin MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title | MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title_full | MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title_fullStr | MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title_full_unstemmed | MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title_short | MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 |
title_sort | mir-708 promotes phagocytosis to eradicate t-all cells by targeting cd47 |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782377/ https://www.ncbi.nlm.nih.gov/pubmed/29368647 http://dx.doi.org/10.1186/s12943-018-0768-2 |
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