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MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47

Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane...

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Autores principales: Huang, Wei, Wang, Wen-Tao, Fang, Ke, Chen, Zhen-Hua, Sun, Yu-Meng, Han, Cai, Sun, Lin-Yu, Luo, Xue-Qun, Chen, Yue-Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782377/
https://www.ncbi.nlm.nih.gov/pubmed/29368647
http://dx.doi.org/10.1186/s12943-018-0768-2
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author Huang, Wei
Wang, Wen-Tao
Fang, Ke
Chen, Zhen-Hua
Sun, Yu-Meng
Han, Cai
Sun, Lin-Yu
Luo, Xue-Qun
Chen, Yue-Qin
author_facet Huang, Wei
Wang, Wen-Tao
Fang, Ke
Chen, Zhen-Hua
Sun, Yu-Meng
Han, Cai
Sun, Lin-Yu
Luo, Xue-Qun
Chen, Yue-Qin
author_sort Huang, Wei
collection PubMed
description Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane protein that inhibits phagocytosis in T cell acute lymphoblastic leukemia. miR-708 directly targeted CD47 through binding to 3’UTR and is inversely correlated with CD47 expression. Functional studies showed that restoration of miR-708 expression in the T-ALL cell line is sufficient to promote phagocytosis by macrophages in the absence or presence of the anti-CD47 antibody to eradicate T-ALL cells, and inhibited tumor engraftment in vivo. Together, our findings suggest that miR-708 is a key negative regulator of CD47 and may serve as an attractive candidate for immunotherapy of T-ALL. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-018-0768-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-57823772018-02-06 MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47 Huang, Wei Wang, Wen-Tao Fang, Ke Chen, Zhen-Hua Sun, Yu-Meng Han, Cai Sun, Lin-Yu Luo, Xue-Qun Chen, Yue-Qin Mol Cancer Letter to the Editor Immunoevasion is a hallmark of cancer progression, and immune checkpoint blockade has emerged as a promising strategy for cancer treatment. microRNAs (miRNAs) are important negative regulators of gene expression in the immune system. Here, we demonstrate that miR-708 regulates CD47, a transmembrane protein that inhibits phagocytosis in T cell acute lymphoblastic leukemia. miR-708 directly targeted CD47 through binding to 3’UTR and is inversely correlated with CD47 expression. Functional studies showed that restoration of miR-708 expression in the T-ALL cell line is sufficient to promote phagocytosis by macrophages in the absence or presence of the anti-CD47 antibody to eradicate T-ALL cells, and inhibited tumor engraftment in vivo. Together, our findings suggest that miR-708 is a key negative regulator of CD47 and may serve as an attractive candidate for immunotherapy of T-ALL. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-018-0768-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-24 /pmc/articles/PMC5782377/ /pubmed/29368647 http://dx.doi.org/10.1186/s12943-018-0768-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Huang, Wei
Wang, Wen-Tao
Fang, Ke
Chen, Zhen-Hua
Sun, Yu-Meng
Han, Cai
Sun, Lin-Yu
Luo, Xue-Qun
Chen, Yue-Qin
MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title_full MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title_fullStr MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title_full_unstemmed MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title_short MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47
title_sort mir-708 promotes phagocytosis to eradicate t-all cells by targeting cd47
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782377/
https://www.ncbi.nlm.nih.gov/pubmed/29368647
http://dx.doi.org/10.1186/s12943-018-0768-2
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