MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1

Many microRNAs (miRs) have been demonstrated to play promoting or tumor suppressive roles in human cancers including breast cancer. However, the molecular mechanism of miR-133a underlying the malignant progression of breast cancer still remains obscure. In the present study we observed that the expr...

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Autores principales: Sui, Yanmin, Zhang, Xiaolei, Yang, Honglan, Wei, Wei, Wang, Minglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783615/
https://www.ncbi.nlm.nih.gov/pubmed/29207145
http://dx.doi.org/10.3892/or.2017.6114
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author Sui, Yanmin
Zhang, Xiaolei
Yang, Honglan
Wei, Wei
Wang, Minglin
author_facet Sui, Yanmin
Zhang, Xiaolei
Yang, Honglan
Wei, Wei
Wang, Minglin
author_sort Sui, Yanmin
collection PubMed
description Many microRNAs (miRs) have been demonstrated to play promoting or tumor suppressive roles in human cancers including breast cancer. However, the molecular mechanism of miR-133a underlying the malignant progression of breast cancer still remains obscure. In the present study we observed that the expression of miR-133a was significantly downregulated in breast cancer tissues and cell lines, when compared with adjacent non-tumor tissues and normal breast cell line, respectively. Reduced miR-133a levels were significantly associated with advanced clinical stage, lymph node metastasis, as well as shorter survival time of patients with breast cancer. Restoration of miR-133a expression led to significant decrease in the proliferation, migration, and invasion of SK-BR-3 and MDA-MB-231 cells in vitro, as well as in tumor xenograft growth in nude mice. Luciferase reporter gene assay data identified LASP1 as a target gene of miR-133a, and the expression of LASP1 was negatively regulated by miR-133a in breast cancer cells. LASP1 was significantly upregulated in breast cancer tissues and cell lines, and its upregulation was significantly associated with disease progression. siRNA-induced LASP1 downregulation caused a significant reduction in breast cancer cell proliferation, migration and invasion. Furthermore, overexpression of LASP1 impaired the suppressive effects of miR-133a upregulation on the proliferation, migration and invasion of SK-BR-3 and MDA-MB-231 cells. In summary, the present study demonstrates that miR-133a acts as a tumor suppressor in breast cancer partly at least via targeting LASP1, and thus suggests that the miR-133a/LASP1 axis may become a potential therapeutic target for breast cancer.
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spelling pubmed-57836152018-02-12 MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1 Sui, Yanmin Zhang, Xiaolei Yang, Honglan Wei, Wei Wang, Minglin Oncol Rep Articles Many microRNAs (miRs) have been demonstrated to play promoting or tumor suppressive roles in human cancers including breast cancer. However, the molecular mechanism of miR-133a underlying the malignant progression of breast cancer still remains obscure. In the present study we observed that the expression of miR-133a was significantly downregulated in breast cancer tissues and cell lines, when compared with adjacent non-tumor tissues and normal breast cell line, respectively. Reduced miR-133a levels were significantly associated with advanced clinical stage, lymph node metastasis, as well as shorter survival time of patients with breast cancer. Restoration of miR-133a expression led to significant decrease in the proliferation, migration, and invasion of SK-BR-3 and MDA-MB-231 cells in vitro, as well as in tumor xenograft growth in nude mice. Luciferase reporter gene assay data identified LASP1 as a target gene of miR-133a, and the expression of LASP1 was negatively regulated by miR-133a in breast cancer cells. LASP1 was significantly upregulated in breast cancer tissues and cell lines, and its upregulation was significantly associated with disease progression. siRNA-induced LASP1 downregulation caused a significant reduction in breast cancer cell proliferation, migration and invasion. Furthermore, overexpression of LASP1 impaired the suppressive effects of miR-133a upregulation on the proliferation, migration and invasion of SK-BR-3 and MDA-MB-231 cells. In summary, the present study demonstrates that miR-133a acts as a tumor suppressor in breast cancer partly at least via targeting LASP1, and thus suggests that the miR-133a/LASP1 axis may become a potential therapeutic target for breast cancer. D.A. Spandidos 2018-02 2017-11-27 /pmc/articles/PMC5783615/ /pubmed/29207145 http://dx.doi.org/10.3892/or.2017.6114 Text en Copyright: © Sui et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sui, Yanmin
Zhang, Xiaolei
Yang, Honglan
Wei, Wei
Wang, Minglin
MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title_full MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title_fullStr MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title_full_unstemmed MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title_short MicroRNA-133a acts as a tumour suppressor in breast cancer through targeting LASP1
title_sort microrna-133a acts as a tumour suppressor in breast cancer through targeting lasp1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783615/
https://www.ncbi.nlm.nih.gov/pubmed/29207145
http://dx.doi.org/10.3892/or.2017.6114
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