A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avia...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Na, Zhang, Yaoyao, Pedrera, Miriam, Chang, Pengxiang, Baigent, Susan, Moffat, Katy, Shen, Zhiqiang, Nair, Venugopal, Yao, Yongxiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783714/
https://www.ncbi.nlm.nih.gov/pubmed/29269155
http://dx.doi.org/10.1016/j.vaccine.2017.12.025
_version_ 1783295326294114304
author Tang, Na
Zhang, Yaoyao
Pedrera, Miriam
Chang, Pengxiang
Baigent, Susan
Moffat, Katy
Shen, Zhiqiang
Nair, Venugopal
Yao, Yongxiu
author_facet Tang, Na
Zhang, Yaoyao
Pedrera, Miriam
Chang, Pengxiang
Baigent, Susan
Moffat, Katy
Shen, Zhiqiang
Nair, Venugopal
Yao, Yongxiu
author_sort Tang, Na
collection PubMed
description Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avian diseases such as infectious bursal disease (IBD), Newcastle disease (ND) and avian influenza (AI) using conventional recombination methods or recombineering tools on cloned viral genomes. In the present study, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of generating HVT recombinants expressing VP2 protein of IBDV. This approach offers an efficient method to introduce other viral antigens into the HVT genome for rapid development of recombinant vaccines.
format Online
Article
Text
id pubmed-5783714
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier Science
record_format MEDLINE/PubMed
spelling pubmed-57837142018-01-31 A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system Tang, Na Zhang, Yaoyao Pedrera, Miriam Chang, Pengxiang Baigent, Susan Moffat, Katy Shen, Zhiqiang Nair, Venugopal Yao, Yongxiu Vaccine Article Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avian diseases such as infectious bursal disease (IBD), Newcastle disease (ND) and avian influenza (AI) using conventional recombination methods or recombineering tools on cloned viral genomes. In the present study, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of generating HVT recombinants expressing VP2 protein of IBDV. This approach offers an efficient method to introduce other viral antigens into the HVT genome for rapid development of recombinant vaccines. Elsevier Science 2018-01-29 /pmc/articles/PMC5783714/ /pubmed/29269155 http://dx.doi.org/10.1016/j.vaccine.2017.12.025 Text en © 2018 The Pirbright Institute http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Na
Zhang, Yaoyao
Pedrera, Miriam
Chang, Pengxiang
Baigent, Susan
Moffat, Katy
Shen, Zhiqiang
Nair, Venugopal
Yao, Yongxiu
A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title_full A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title_fullStr A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title_full_unstemmed A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title_short A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system
title_sort simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using crispr/cas9 system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783714/
https://www.ncbi.nlm.nih.gov/pubmed/29269155
http://dx.doi.org/10.1016/j.vaccine.2017.12.025
work_keys_str_mv AT tangna asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT zhangyaoyao asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT pedreramiriam asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT changpengxiang asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT baigentsusan asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT moffatkaty asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT shenzhiqiang asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT nairvenugopal asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT yaoyongxiu asimpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT tangna simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT zhangyaoyao simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT pedreramiriam simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT changpengxiang simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT baigentsusan simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT moffatkaty simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT shenzhiqiang simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT nairvenugopal simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system
AT yaoyongxiu simpleandrapidapproachtodeveloprecombinantavianherpesvirusvectoredvaccinesusingcrisprcas9system

Ejemplares similares