Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment

Backbone resonance assignment is a critical first step in the investigation of proteins by NMR. This is traditionally achieved with a standard set of experiments, most of which are not optimal for large proteins. Of these, HNCA is the most sensitive experiment that provides sequential correlations....

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Detalles Bibliográficos
Autores principales: Robson, Scott A., Takeuchi, Koh, Boeszoermenyi, Andras, Coote, Paul W., Dubey, Abhinav, Hyberts, Sven, Wagner, Gerhard, Arthanari, Haribabu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783931/
https://www.ncbi.nlm.nih.gov/pubmed/29367739
http://dx.doi.org/10.1038/s41467-017-02767-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783931/
https://www.ncbi.nlm.nih.gov/pubmed/29367739
http://dx.doi.org/10.1038/s41467-017-02767-8

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