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Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment
Backbone resonance assignment is a critical first step in the investigation of proteins by NMR. This is traditionally achieved with a standard set of experiments, most of which are not optimal for large proteins. Of these, HNCA is the most sensitive experiment that provides sequential correlations....
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783931/ https://www.ncbi.nlm.nih.gov/pubmed/29367739 http://dx.doi.org/10.1038/s41467-017-02767-8 |