Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms

MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants (“isomiRs”), little attention has been paid t...

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Detalles Bibliográficos
Autores principales: Nejad, Charlotte, Pépin, Geneviève, Behlke, Mark A., Gantier, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787544/
https://www.ncbi.nlm.nih.gov/pubmed/29416548
http://dx.doi.org/10.3389/fgene.2018.00011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787544/
https://www.ncbi.nlm.nih.gov/pubmed/29416548
http://dx.doi.org/10.3389/fgene.2018.00011

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