Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments. We show that CRISPR‐Cas9‐mediated gene pertur...

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Detalles Bibliográficos
Autores principales: de Groot, Reinoud, Lüthi, Joel, Lindsay, Helen, Holtackers, René, Pelkmans, Lucas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787707/
https://www.ncbi.nlm.nih.gov/pubmed/29363560
http://dx.doi.org/10.15252/msb.20178064

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787707/
https://www.ncbi.nlm.nih.gov/pubmed/29363560
http://dx.doi.org/10.15252/msb.20178064

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