Efficient strategy for introducing large and multiple changes in plasmid DNA

While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligation of Fragment Ends After PCR) for creating various mutations in plasmid by leveraging...

Descripción completa

Detalles Bibliográficos
Autores principales: Zeng, Fanli, Zhang, Suhua, Hao, Zhimin, Duan, Shixin, Meng, Yanan, Li, Pan, Dong, Jingao, Lin, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789069/
https://www.ncbi.nlm.nih.gov/pubmed/29379085
http://dx.doi.org/10.1038/s41598-018-20169-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789069/
https://www.ncbi.nlm.nih.gov/pubmed/29379085
http://dx.doi.org/10.1038/s41598-018-20169-8

Ejemplares similares