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Reagent Tracker Dyes Permit Quality Control for Verifying Plating Accuracy in ELISPOT Tests

ELISPOT assays enable the detection of the frequency of antigen-specific T cells in the blood by measuring the secretion of cytokines, or combinations of cytokines, in response to antigenic challenges of a defined population of PBMC. As such, these assays are suited to establish the magnitude and qu...

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Detalles Bibliográficos
Autores principales: Lehmann, Alexander, Megyesi, Zoltan, Przybyla, Anna, Lehmann, Paul V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789276/
https://www.ncbi.nlm.nih.gov/pubmed/29301355
http://dx.doi.org/10.3390/cells7010003