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Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. Th...

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Detalles Bibliográficos
Autores principales: Feng, Na, Zhou, Yazhou, Fan, Yanxiao, Bi, Yujing, Yang, Ruifu, Zhou, Yusen, Wang, Xiaoyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790586/
https://www.ncbi.nlm.nih.gov/pubmed/28887007
http://dx.doi.org/10.1016/j.bjm.2017.03.014