Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability

[Image: see text] For years, the use of polyhistidine tags (His-tags) has been a staple in the isolation of recombinant proteins in immobilized metal affinity chromatography experiments. Their usage has been widely beneficial in increasing protein purity from crude cell lysates. For some recombinant...

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Detalles Bibliográficos
Autores principales: Booth, William T., Schlachter, Caleb R., Pote, Swanandi, Ussin, Nikita, Mank, Nicholas J., Klapper, Vincent, Offermann, Lesa R., Tang, Chuanbing, Hurlburt, Barry K., Chruszcz, Maksymilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793033/
https://www.ncbi.nlm.nih.gov/pubmed/29399652
http://dx.doi.org/10.1021/acsomega.7b01598

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793033/
https://www.ncbi.nlm.nih.gov/pubmed/29399652
http://dx.doi.org/10.1021/acsomega.7b01598

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