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Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability

[Image: see text] For years, the use of polyhistidine tags (His-tags) has been a staple in the isolation of recombinant proteins in immobilized metal affinity chromatography experiments. Their usage has been widely beneficial in increasing protein purity from crude cell lysates. For some recombinant...

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Detalles Bibliográficos
Autores principales:	Booth, William T., Schlachter, Caleb R., Pote, Swanandi, Ussin, Nikita, Mank, Nicholas J., Klapper, Vincent, Offermann, Lesa R., Tang, Chuanbing, Hurlburt, Barry K., Chruszcz, Maksymilian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2018
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793033/ https://www.ncbi.nlm.nih.gov/pubmed/29399652 http://dx.doi.org/10.1021/acsomega.7b01598

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