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Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes

Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences. Here, we describe Cleaning and Co-assembly of a Single-Cell Amplified Genome (ccSAG), a no...

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Detalles Bibliográficos
Autores principales: Kogawa, Masato, Hosokawa, Masahito, Nishikawa, Yohei, Mori, Kazuki, Takeyama, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794965/
https://www.ncbi.nlm.nih.gov/pubmed/29391438
http://dx.doi.org/10.1038/s41598-018-20384-3