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Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum

Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments. However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses....

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Detalles Bibliográficos
Autores principales: Kornilov, Roman, Puhka, Maija, Mannerström, Bettina, Hiidenmaa, Hanna, Peltoniemi, Hilkka, Siljander, Pia, Seppänen-Kaijansinkko, Riitta, Kaur, Sippy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795649/
https://www.ncbi.nlm.nih.gov/pubmed/29410778
http://dx.doi.org/10.1080/20013078.2017.1422674