Keeping it real: MRX–Sae2 clipping of natural substrates

The yeast Mre11–Rad50–Xrs2 (MRX) complex and Sae2 function together to initiate DNA end resection, an essential early step in homology-dependent repair of DNA double-strand breaks (DSBs). In this issue of Genes & Development, Wang and colleagues (pp. 2331–2336) and Reginato and colleagues (pp. 2...

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Detalles Bibliográficos
Autores principales: Gnügge, Robert, Symington, Lorraine S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Outlook
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795777/
https://www.ncbi.nlm.nih.gov/pubmed/29352017
http://dx.doi.org/10.1101/gad.310771.117
Descripción
Sumario:The yeast Mre11–Rad50–Xrs2 (MRX) complex and Sae2 function together to initiate DNA end resection, an essential early step in homology-dependent repair of DNA double-strand breaks (DSBs). In this issue of Genes & Development, Wang and colleagues (pp. 2331–2336) and Reginato and colleagues (pp. 2325–2330) report that a variety of physiological protein blocks, including Ku, RPA, and nucleosomes, stimulate MRX–Sae2 endonuclease cleavage in vitro. These studies have important implications for how cells deal with a range of barriers to end resection and highlight the crucial role of Sae2 in activating MRX cleavage at the correct cell cycle stage.

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