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Evaluation of reference genes for gene expression studies in mouse and N2a cell ischemic stroke models using quantitative real-time PCR

BACKGROUND: Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a critical tool for evaluating the levels of mRNA transcribed from genes. Reliable RT-qPCR results largely depend on normalization to suitable reference genes. Middle cerebral artery occlusion (MCAO) and...

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Detalles Bibliográficos
Autores principales: Kang, Yingbo, Wu, Zhuomin, Cai, De, Lu, Binger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795833/
https://www.ncbi.nlm.nih.gov/pubmed/29390963
http://dx.doi.org/10.1186/s12868-018-0403-6