Cargando…

Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador

Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical...

Descripción completa

Detalles Bibliográficos
Autores principales: García-Tenesaca, Marilyn, Navarrete, Eillen S., Iturralde, Gabriel A., Villacrés Granda, Irina M., Tejera, Eduardo, Beltrán-Ayala, Pablo, Giampieri, Francesca, Battino, Maurizio, Alvarez-Suarez, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795995/
https://www.ncbi.nlm.nih.gov/pubmed/29295525
http://dx.doi.org/10.3390/ijms19010045
_version_ 1783297408286851072
author García-Tenesaca, Marilyn
Navarrete, Eillen S.
Iturralde, Gabriel A.
Villacrés Granda, Irina M.
Tejera, Eduardo
Beltrán-Ayala, Pablo
Giampieri, Francesca
Battino, Maurizio
Alvarez-Suarez, José M.
author_facet García-Tenesaca, Marilyn
Navarrete, Eillen S.
Iturralde, Gabriel A.
Villacrés Granda, Irina M.
Tejera, Eduardo
Beltrán-Ayala, Pablo
Giampieri, Francesca
Battino, Maurizio
Alvarez-Suarez, José M.
author_sort García-Tenesaca, Marilyn
collection PubMed
description Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical composition varied considerably depending on floral origin. The highest values of bioactive compounds were found in Avocado honey, classified as dark amber in color, while the lowest values were found in Eucalyptus honey followed by Rapeseed honey, both classified as extra light amber. When compared to Eucalyptus and Rapeseed honey, Avocado honey showed a more effective superoxide scavenging activity, chelating metal ions capacity, and a higher ability to protect human erythrocyte membranes against lipid peroxidation. For antimicrobial activity, the hydrogen peroxide content and the capacity to inhibit the biofilm formation, and to remove preformed biofilm from Staphylococcus aureus and Klebsiella pneumoniae was determined. Avocado honey showed the highest values of hydrogen peroxide content, as well as the highest capacity to reduce in vitro bacterial biofilms. A correlation between color vs. phenolics content vs. superoxide scavenging activity vs. chelating metal ions capacity, and the capacity to protect human erythrocyte membranes against lipid peroxidation was found.
format Online
Article
Text
id pubmed-5795995
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57959952018-02-09 Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador García-Tenesaca, Marilyn Navarrete, Eillen S. Iturralde, Gabriel A. Villacrés Granda, Irina M. Tejera, Eduardo Beltrán-Ayala, Pablo Giampieri, Francesca Battino, Maurizio Alvarez-Suarez, José M. Int J Mol Sci Article Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical composition varied considerably depending on floral origin. The highest values of bioactive compounds were found in Avocado honey, classified as dark amber in color, while the lowest values were found in Eucalyptus honey followed by Rapeseed honey, both classified as extra light amber. When compared to Eucalyptus and Rapeseed honey, Avocado honey showed a more effective superoxide scavenging activity, chelating metal ions capacity, and a higher ability to protect human erythrocyte membranes against lipid peroxidation. For antimicrobial activity, the hydrogen peroxide content and the capacity to inhibit the biofilm formation, and to remove preformed biofilm from Staphylococcus aureus and Klebsiella pneumoniae was determined. Avocado honey showed the highest values of hydrogen peroxide content, as well as the highest capacity to reduce in vitro bacterial biofilms. A correlation between color vs. phenolics content vs. superoxide scavenging activity vs. chelating metal ions capacity, and the capacity to protect human erythrocyte membranes against lipid peroxidation was found. MDPI 2017-12-23 /pmc/articles/PMC5795995/ /pubmed/29295525 http://dx.doi.org/10.3390/ijms19010045 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Tenesaca, Marilyn
Navarrete, Eillen S.
Iturralde, Gabriel A.
Villacrés Granda, Irina M.
Tejera, Eduardo
Beltrán-Ayala, Pablo
Giampieri, Francesca
Battino, Maurizio
Alvarez-Suarez, José M.
Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title_full Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title_fullStr Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title_full_unstemmed Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title_short Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador
title_sort influence of botanical origin and chemical composition on the protective effect against oxidative damage and the capacity to reduce in vitro bacterial biofilms of monofloral honeys from the andean region of ecuador
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795995/
https://www.ncbi.nlm.nih.gov/pubmed/29295525
http://dx.doi.org/10.3390/ijms19010045
work_keys_str_mv AT garciatenesacamarilyn influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT navarreteeillens influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT iturraldegabriela influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT villacresgrandairinam influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT tejeraeduardo influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT beltranayalapablo influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT giampierifrancesca influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT battinomaurizio influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador
AT alvarezsuarezjosem influenceofbotanicaloriginandchemicalcompositionontheprotectiveeffectagainstoxidativedamageandthecapacitytoreduceinvitrobacterialbiofilmsofmonofloralhoneysfromtheandeanregionofecuador