Skin Protective Effect of Epigallocatechin Gallate

Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuri...

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Autores principales: Kim, Eunji, Hwang, Kyeonghwan, Lee, Jongsung, Han, Sang Yun, Kim, Eun-Mi, Park, Junseong, Cho, Jae Youl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796122/
https://www.ncbi.nlm.nih.gov/pubmed/29316635
http://dx.doi.org/10.3390/ijms19010173
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author Kim, Eunji
Hwang, Kyeonghwan
Lee, Jongsung
Han, Sang Yun
Kim, Eun-Mi
Park, Junseong
Cho, Jae Youl
author_facet Kim, Eunji
Hwang, Kyeonghwan
Lee, Jongsung
Han, Sang Yun
Kim, Eun-Mi
Park, Junseong
Cho, Jae Youl
author_sort Kim, Eunji
collection PubMed
description Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase-1, HAS-1, and HAS-2. Under UVB irradiation conditions, the expression level of HYAL was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of EGCG and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells. EGCG reduced melanin secretion and production in melanoma cells. Together, these results suggest that EGCG might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation.
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spelling pubmed-57961222018-02-09 Skin Protective Effect of Epigallocatechin Gallate Kim, Eunji Hwang, Kyeonghwan Lee, Jongsung Han, Sang Yun Kim, Eun-Mi Park, Junseong Cho, Jae Youl Int J Mol Sci Article Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase-1, HAS-1, and HAS-2. Under UVB irradiation conditions, the expression level of HYAL was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of EGCG and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells. EGCG reduced melanin secretion and production in melanoma cells. Together, these results suggest that EGCG might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation. MDPI 2018-01-06 /pmc/articles/PMC5796122/ /pubmed/29316635 http://dx.doi.org/10.3390/ijms19010173 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Eunji
Hwang, Kyeonghwan
Lee, Jongsung
Han, Sang Yun
Kim, Eun-Mi
Park, Junseong
Cho, Jae Youl
Skin Protective Effect of Epigallocatechin Gallate
title Skin Protective Effect of Epigallocatechin Gallate
title_full Skin Protective Effect of Epigallocatechin Gallate
title_fullStr Skin Protective Effect of Epigallocatechin Gallate
title_full_unstemmed Skin Protective Effect of Epigallocatechin Gallate
title_short Skin Protective Effect of Epigallocatechin Gallate
title_sort skin protective effect of epigallocatechin gallate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796122/
https://www.ncbi.nlm.nih.gov/pubmed/29316635
http://dx.doi.org/10.3390/ijms19010173
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