Skin Protective Effect of Epigallocatechin Gallate
Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuri...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796122/ https://www.ncbi.nlm.nih.gov/pubmed/29316635 http://dx.doi.org/10.3390/ijms19010173 |
_version_ | 1783297438055923712 |
---|---|
author | Kim, Eunji Hwang, Kyeonghwan Lee, Jongsung Han, Sang Yun Kim, Eun-Mi Park, Junseong Cho, Jae Youl |
author_facet | Kim, Eunji Hwang, Kyeonghwan Lee, Jongsung Han, Sang Yun Kim, Eun-Mi Park, Junseong Cho, Jae Youl |
author_sort | Kim, Eunji |
collection | PubMed |
description | Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase-1, HAS-1, and HAS-2. Under UVB irradiation conditions, the expression level of HYAL was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of EGCG and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells. EGCG reduced melanin secretion and production in melanoma cells. Together, these results suggest that EGCG might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation. |
format | Online Article Text |
id | pubmed-5796122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57961222018-02-09 Skin Protective Effect of Epigallocatechin Gallate Kim, Eunji Hwang, Kyeonghwan Lee, Jongsung Han, Sang Yun Kim, Eun-Mi Park, Junseong Cho, Jae Youl Int J Mol Sci Article Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase-1, HAS-1, and HAS-2. Under UVB irradiation conditions, the expression level of HYAL was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of EGCG and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells. EGCG reduced melanin secretion and production in melanoma cells. Together, these results suggest that EGCG might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation. MDPI 2018-01-06 /pmc/articles/PMC5796122/ /pubmed/29316635 http://dx.doi.org/10.3390/ijms19010173 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Eunji Hwang, Kyeonghwan Lee, Jongsung Han, Sang Yun Kim, Eun-Mi Park, Junseong Cho, Jae Youl Skin Protective Effect of Epigallocatechin Gallate |
title | Skin Protective Effect of Epigallocatechin Gallate |
title_full | Skin Protective Effect of Epigallocatechin Gallate |
title_fullStr | Skin Protective Effect of Epigallocatechin Gallate |
title_full_unstemmed | Skin Protective Effect of Epigallocatechin Gallate |
title_short | Skin Protective Effect of Epigallocatechin Gallate |
title_sort | skin protective effect of epigallocatechin gallate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796122/ https://www.ncbi.nlm.nih.gov/pubmed/29316635 http://dx.doi.org/10.3390/ijms19010173 |
work_keys_str_mv | AT kimeunji skinprotectiveeffectofepigallocatechingallate AT hwangkyeonghwan skinprotectiveeffectofepigallocatechingallate AT leejongsung skinprotectiveeffectofepigallocatechingallate AT hansangyun skinprotectiveeffectofepigallocatechingallate AT kimeunmi skinprotectiveeffectofepigallocatechingallate AT parkjunseong skinprotectiveeffectofepigallocatechingallate AT chojaeyoul skinprotectiveeffectofepigallocatechingallate |