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Preservation Method and Phosphate Buffered Saline Washing Affect the Acute Myeloid Leukemia Proteome

Acute myeloid leukemia (AML) primary cells can be isolated from peripheral blood, suspended with media containing bovine serum and cryoprotectant, and stored in liquid nitrogen before being processed for proteomic analysis by mass spectrometry (MS). The presence of bovine serum and human blood prote...

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Detalles Bibliográficos
Autores principales: Wangen, Rebecca, Aasebø, Elise, Trentani, Andrea, Døskeland, Stein-Ove, Bruserud, Øystein, Selheim, Frode, Hernandez-Valladares, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796241/
https://www.ncbi.nlm.nih.gov/pubmed/29351208
http://dx.doi.org/10.3390/ijms19010296