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Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungus Pecoramyces ruminantium strain C1A

Members of the anaerobic gut fungi (AGF) reside in rumen, hindgut, and feces of ruminant and non-ruminant herbivorous mammals and reptilian herbivores. No protocols for gene insertion, deletion, silencing, or mutation are currently available for the AGF, rendering gene-targeted molecular biological...

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Detalles Bibliográficos
Autores principales: Calkins, Shelby S., Elledge, Nicole C., Mueller, Katherine E., Marek, Stephen M., Couger, MB, Elshahed, Mostafa S., Youssef, Noha H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796279/
https://www.ncbi.nlm.nih.gov/pubmed/29404209
http://dx.doi.org/10.7717/peerj.4276