Cargando…
Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
BACKGROUND: Medaka (Oryzias latipes) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka, it woul...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798193/ https://www.ncbi.nlm.nih.gov/pubmed/29445519 http://dx.doi.org/10.1186/s40851-017-0086-3 |