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Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering

BACKGROUND: Medaka (Oryzias latipes) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka, it woul...

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Detalles Bibliográficos
Autores principales:	Watakabe, Ikuko, Hashimoto, Hisashi, Kimura, Yukiko, Yokoi, Saori, Naruse, Kiyoshi, Higashijima, Shin-ichi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798193/ https://www.ncbi.nlm.nih.gov/pubmed/29445519 http://dx.doi.org/10.1186/s40851-017-0086-3

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