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Systematic comparison of small RNA library preparation protocols for next-generation sequencing

BACKGROUND: Next-generation sequencing technologies have revolutionized the study of small RNAs (sRNAs) on a genome-wide scale. However, classical sRNA library preparation methods introduce serious bias, mainly during adapter ligation steps. Several types of sRNA including plant microRNAs (miRNA), p...

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Detalles Bibliográficos
Autores principales: Dard-Dascot, Cloelia, Naquin, Delphine, d’Aubenton-Carafa, Yves, Alix, Karine, Thermes, Claude, van Dijk, Erwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799908/
https://www.ncbi.nlm.nih.gov/pubmed/29402217
http://dx.doi.org/10.1186/s12864-018-4491-6