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Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker

We developed a novel optogenetic tool, SxIP–improved light-inducible dimer (iLID), to facilitate the reversible recruitment of factors to microtubule (MT) plus ends in an end-binding protein–dependent manner using blue light. We show that SxIP-iLID can track MT plus ends and recruit tgRFP-SspB upon...

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Autores principales: Adikes, Rebecca C., Hallett, Ryan A., Saway, Brian F., Kuhlman, Brian, Slep, Kevin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800807/
https://www.ncbi.nlm.nih.gov/pubmed/29259096
http://dx.doi.org/10.1083/jcb.201705190
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author Adikes, Rebecca C.
Hallett, Ryan A.
Saway, Brian F.
Kuhlman, Brian
Slep, Kevin C.
author_facet Adikes, Rebecca C.
Hallett, Ryan A.
Saway, Brian F.
Kuhlman, Brian
Slep, Kevin C.
author_sort Adikes, Rebecca C.
collection PubMed
description We developed a novel optogenetic tool, SxIP–improved light-inducible dimer (iLID), to facilitate the reversible recruitment of factors to microtubule (MT) plus ends in an end-binding protein–dependent manner using blue light. We show that SxIP-iLID can track MT plus ends and recruit tgRFP-SspB upon blue light activation. We used this system to investigate the effects of cross-linking MT plus ends and F-actin in Drosophila melanogaster S2 cells to gain insight into spectraplakin function and mechanism. We show that SxIP-iLID can be used to temporally recruit an F-actin binding domain to MT plus ends and cross-link the MT and F-actin networks. Cross-linking decreases MT growth velocities and generates a peripheral MT exclusion zone. SxIP-iLID facilitates the general recruitment of specific factors to MT plus ends with temporal control enabling researchers to systematically regulate MT plus end dynamics and probe MT plus end function in many biological processes.
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spelling pubmed-58008072018-08-05 Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker Adikes, Rebecca C. Hallett, Ryan A. Saway, Brian F. Kuhlman, Brian Slep, Kevin C. J Cell Biol Research Articles We developed a novel optogenetic tool, SxIP–improved light-inducible dimer (iLID), to facilitate the reversible recruitment of factors to microtubule (MT) plus ends in an end-binding protein–dependent manner using blue light. We show that SxIP-iLID can track MT plus ends and recruit tgRFP-SspB upon blue light activation. We used this system to investigate the effects of cross-linking MT plus ends and F-actin in Drosophila melanogaster S2 cells to gain insight into spectraplakin function and mechanism. We show that SxIP-iLID can be used to temporally recruit an F-actin binding domain to MT plus ends and cross-link the MT and F-actin networks. Cross-linking decreases MT growth velocities and generates a peripheral MT exclusion zone. SxIP-iLID facilitates the general recruitment of specific factors to MT plus ends with temporal control enabling researchers to systematically regulate MT plus end dynamics and probe MT plus end function in many biological processes. The Rockefeller University Press 2018-02-05 /pmc/articles/PMC5800807/ /pubmed/29259096 http://dx.doi.org/10.1083/jcb.201705190 Text en © 2018 Adikes et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Adikes, Rebecca C.
Hallett, Ryan A.
Saway, Brian F.
Kuhlman, Brian
Slep, Kevin C.
Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title_full Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title_fullStr Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title_full_unstemmed Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title_short Control of microtubule dynamics using an optogenetic microtubule plus end–F-actin cross-linker
title_sort control of microtubule dynamics using an optogenetic microtubule plus end–f-actin cross-linker
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800807/
https://www.ncbi.nlm.nih.gov/pubmed/29259096
http://dx.doi.org/10.1083/jcb.201705190
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