Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice

A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing β3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG(3) subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding...

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Detalles Bibliográficos
Autores principales: He, Dongwei, Fan, Xiaoyan, Liu, Boyi, Tian, Yiqing, Zhang, Xiangmei, Kang, Lin, Tai, Yan, Liu, Shuzhen, Wang, Qian, Li, Qingxia, Cai, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803271/
https://www.ncbi.nlm.nih.gov/pubmed/29416099
http://dx.doi.org/10.1038/s41598-018-20951-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803271/
https://www.ncbi.nlm.nih.gov/pubmed/29416099
http://dx.doi.org/10.1038/s41598-018-20951-8

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