Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice

A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing β3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG(3) subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding...

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Autores principales: He, Dongwei, Fan, Xiaoyan, Liu, Boyi, Tian, Yiqing, Zhang, Xiangmei, Kang, Lin, Tai, Yan, Liu, Shuzhen, Wang, Qian, Li, Qingxia, Cai, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803271/
https://www.ncbi.nlm.nih.gov/pubmed/29416099
http://dx.doi.org/10.1038/s41598-018-20951-8
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author He, Dongwei
Fan, Xiaoyan
Liu, Boyi
Tian, Yiqing
Zhang, Xiangmei
Kang, Lin
Tai, Yan
Liu, Shuzhen
Wang, Qian
Li, Qingxia
Cai, Jianhui
author_facet He, Dongwei
Fan, Xiaoyan
Liu, Boyi
Tian, Yiqing
Zhang, Xiangmei
Kang, Lin
Tai, Yan
Liu, Shuzhen
Wang, Qian
Li, Qingxia
Cai, Jianhui
author_sort He, Dongwei
collection PubMed
description A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing β3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG(3) subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding specificity with GM3. Cell viability assay showed that MAb-1 significantly suppressed cell growth. Immunohistochemistry analysis revealed that MAb-1 was strongly expressed in human ovarian cancer tissues, whereas it was hardly expressed in normal tissues. Finally, antibody-dependent cellular cytotoxicity (ADCC) activities were determined by measuring lactate dehydrogenase (LDH) releasing assay and the results showed high ADCC activities in two representative ovarian cancer cell lines (OVHM and ID8). All of these data indicate that MAb-1 may be potentially used as a therapeutic antibody against ovarian cancers in clinical trials.
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spelling pubmed-58032712018-02-16 Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice He, Dongwei Fan, Xiaoyan Liu, Boyi Tian, Yiqing Zhang, Xiangmei Kang, Lin Tai, Yan Liu, Shuzhen Wang, Qian Li, Qingxia Cai, Jianhui Sci Rep Article A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing β3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG(3) subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding specificity with GM3. Cell viability assay showed that MAb-1 significantly suppressed cell growth. Immunohistochemistry analysis revealed that MAb-1 was strongly expressed in human ovarian cancer tissues, whereas it was hardly expressed in normal tissues. Finally, antibody-dependent cellular cytotoxicity (ADCC) activities were determined by measuring lactate dehydrogenase (LDH) releasing assay and the results showed high ADCC activities in two representative ovarian cancer cell lines (OVHM and ID8). All of these data indicate that MAb-1 may be potentially used as a therapeutic antibody against ovarian cancers in clinical trials. Nature Publishing Group UK 2018-02-07 /pmc/articles/PMC5803271/ /pubmed/29416099 http://dx.doi.org/10.1038/s41598-018-20951-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
He, Dongwei
Fan, Xiaoyan
Liu, Boyi
Tian, Yiqing
Zhang, Xiangmei
Kang, Lin
Tai, Yan
Liu, Shuzhen
Wang, Qian
Li, Qingxia
Cai, Jianhui
Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title_full Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title_fullStr Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title_full_unstemmed Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title_short Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing β3Gn-T5 knockout mice
title_sort generation and characterization of a igg monoclonal antibody specific for gm3 (neugc) ganglioside by immunizing β3gn-t5 knockout mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803271/
https://www.ncbi.nlm.nih.gov/pubmed/29416099
http://dx.doi.org/10.1038/s41598-018-20951-8
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