Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

The existence of cytosine methylation in mammalian mitochondrial DNA (mtDNA) is a controversial subject. Because detection of DNA methylation depends on resistance of 5’-modified cytosines to bisulfite-catalyzed conversion to uracil, examined parameters that affect technical adequacy of mtDNA methyl...

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Detalles Bibliográficos
Autores principales: Owa, Chie, Poulin, Matthew, Yan, Liying, Shioda, Toshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805350/
https://www.ncbi.nlm.nih.gov/pubmed/29420656
http://dx.doi.org/10.1371/journal.pone.0192722

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805350/
https://www.ncbi.nlm.nih.gov/pubmed/29420656
http://dx.doi.org/10.1371/journal.pone.0192722

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