Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds

Genetic circuit-based biosensors are useful in detecting target metabolites or in vivo enzymes using transcription factors (Tx) as a molecular switch to express reporter signals, such as cellular fluorescence and antibiotic resistance. Herein, a phenol-detecting Tx (DmpR) was employed as a critical...

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Detalles Bibliográficos
Autores principales: Kwon, Kil Koang, Lee, Dae-Hee, Kim, Su Jin, Choi, Su-Lim, Rha, Eugene, Yeom, Soo-Jin, Subhadra, Bindu, Lee, Jinhyuk, Jeong, Ki Jun, Lee, Seung-Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805759/
https://www.ncbi.nlm.nih.gov/pubmed/29422524
http://dx.doi.org/10.1038/s41598-018-20943-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805759/
https://www.ncbi.nlm.nih.gov/pubmed/29422524
http://dx.doi.org/10.1038/s41598-018-20943-8

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